Links to Servers



This page contains links to servers which you will use for the
Tools and Comparative Modelling practical
and for the Mini-Projects.



Sequence Searching (Primary databases)

Link Description
BLAST Sequence database search at the NCBI
Local BLAST Sequence database search running locally
FASTA FASTA sequence database search

Motif Searching (Secondary databases)

Link Description
InterPro Integrated resource of secondary databases
PRINTS PRINTS secondary database
BLOCKS BLOCKS secondary database
ProSite ProSite secondary database
Pfam Pfam secondary database (also at Washington University)
MOTIF Integrated motif searching resource

DNA to Protein Translation

Link Description
From manchester... 6-frame translation software
From Expasy 6-frame translation software
From EBI 6-frame translation software

Secondary Structure Prediction

Link Description
NNPredict Neural network prediction of secondary structure
JPRED Secondary structure prediction
GOR GOR SS Prediction
GOR-II/III GOR-II/III SS Prediction
GOR-IV GOR-IV SS Prediction
PSIPred / MemSat PSIPred SS Prediction - Also MemSat TM prediction

Transmembrane Prediction

Link Description
TSEG Predict membrane proteins
TMHMM-2.0 TM prediction through Hidden Markov Models
TopPred Predict location and orientation of transmembrane helices through an advanced use of hydrophobicity patterns, and by applying the 'positive-inside' rule
DAS TM Prediction through an advanced use of hydrophobicity patterns
PRED-TMR TM prediction

Post-translational Modification Prediction

Link Description
NetPhos NetPhos is a neural network-based method for predicting potential phosphorylation sites at serine, threonine or tyrosine residues in protein sequences.
NetOGlyc The NetOglyc WWW server produces neural network predictions of mucin type GalNAc O-glycosylation sites in mammalian proteins.
big-PI GPI Modification Site Prediction
GPI-anchor Another tool for GPI cleavage site (GPI-anchor) prediction

Threading and Fold Recognition

Link Description
FRSVR Daniel Fischer's Prediction-based threading, also incorporating purely sequence-based database searches (new server here)
SAMt98 A new HMM based method to find remote homologs. Beta version of SAM-T99
3D-pssm Fold recognition

Comparative Modelling Servers

Link Description
Swiss-Model an Automated Protein Modelling Server running at the GlaxoWellcome Experimental Research in Geneva, Switzerland
CPHmodels A neural net based comparative modelling tool
CYSPRED Disulphide prediction

Sequence Alignment

Link Description
ClustalW Multiple sequence alignment (also at EMBNet).

Sequence-based Profiles

Link Description
Physico- chemical properties Plot Amino Acid Physico-chemical Properties (hydropathy, predicted accessibility, flexibility, GOR-SS)
ProtScale Plot various sequence-based profiles (hydrophobicity, secondary structure, etc.)

Signal-Peptide Prediction

Link Description
PSORT Predict protein sorting signals
SignalP Signal peptides

Integrated Prediction Systems

Link Description
PredictProtein Prediction system from the Columbia University (also available at the EBI) Includes PHD secondary structure prediction, ProSite, domain prediction, threading, solvent accessibility, TM, etc.